Antibody Assessment Panels

For a comprehensive look at the body’s immune reaction to foods and inhalants, US BioTek Laboratories offers the following antibody assays:

     IgE Antibody Assessment

     IgG Antibody Assessment

     IgA Antibody Assessment

Allergic and other hypersensitivity reactions to foods and aeroallergens are characterized by elevated allergen-specific antibody levels in the affected individual's blood. Research indicates that these types of reactions are implicated in a number of health problems. Through our Enzyme Linked Immunosorbent Assay (ELISA) methodology we provide a useful tool for the practitioner to assess his or her patient's allergies and sensitivities to a variety of common allergens.

Through the ELISA method a multi-well micro titer plate is pre-coated, or adsorbed, with purified protein and glycoprotein allergens at specific concentrations. Our standard curve based method for our semi-quantitative allergen-specific IgE ELISA is traceable to World Health Organization (WHO)’s standards. WHO’s standards increase immunoassay sensitivity against a broad range of allergen concentrations allowing for the detection of minute levels of IgE antibodies in a serum specimen for accurate assay performance. With this US BioTek testing remains superior and competitively positioned in the industry.

A serum specimen is added to the micro titer plate. If the serum specimen contains antibody to any of the specific protein or glycoproteins adhered to the wells of the plate, a binding reaction will occur. This is a selective binding reaction to detect the presence of antibody in a serum specimen that is specific to a defined protein. The degree of antibody-antigen binding is dependent on the concentration of antibody present in the serum specimen. This binding reaction is detected through a secondary antibody linked to an enzyme tag. The plate is developed by adding a chromogenic enzyme substrate that produces a readable signal, or color change. The intensity of the color change indicates the quantity of antibody in the serum specimen. This reaction is assessed spectrophotometrically.

Widely used in many laboratory techniques the ELISA method is a proven sensitive immunoassay to detect the presence of antibody in a specimen.

• Antigen Standardization - We start with the highest quality standardized reagents available from major licensed manufactures in the U.S.A.
  
  
• Accurate and Consistent - We employ high-performance, high-capacity automation and tracking to ensure accurate, uniform, and consistent assay results. Day- to-day assay variability due to technical error is eliminated.
  
  
• All Results are Run in DUPLICATE - This ensures a valid and precise assay with repeatable test results.
  
  
• Our Assay Quantifies True Antibody Levels - We utilize 3 internal controls and 2 negative blanking controls for each assay batch of patient specimens to ensure accurate results.
  
  
• 7-Zone Color Bar Graph - Patient antibody levels against respective antigens for our food and inhalant panels offer a visual representation of the results.
  
  
• Foods and Aeroallergens are grouped by Type. (E.g. dairy, nuts/grains, molds, pollens, etc.)
  
  
• User-Friendly Report within 5-10 days of receiving Blood Specimen.
  
  
• Individualized FoodStats Elimination & Rotation Diet Guideline - Included with each food antibody report exhibiting high reactions.
  
  
• Hassle-free Serum Specimen Pick-up by pre-paid 2nd Day FedEx Service – For the 48 contiguous U.S.A. states. Safely shipped at room temperature.
  
  
• Hassle-free pre-paid USPS shipping for Dried Blood Spot Specimens – For ALL U.S.A. states. Safely shipped at room temperature.

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