GI - Advanced Profile

The GI – Advanced Profile is the most comprehensive GI Microbiome Profile building upon the Basic and Standard profile to offer 98 markers from 15 different testing categories. The profile combines different testing methodologies to offer highly sensitive results with industry-leading accuracy and reproducibility.

Key Phyla % Commensal Abundance

  • Bacteriodetes
  • Firmicutes
  • Firm/Bact Ratio
  • Proteobacteria
  • Actinobacteria
  • Verrucomicrobia
  • Euryarchaeota

Parasites & Worms

  • Cryptosporidium
  • Ent. Histolytica
  • Giardia Intestinalis
  • Blast. hominis
  • Dient. Fragilis
  • Endolimax nana
  • Ent. Coli
  • Pent. Hominis
  • Ascaris lumbricoides, round
  • Necator americanus,hook
  • Trichuris trichuria, round
  • Taenia species, tape
  • Enterob. Vermicularis
  • Strongyloides stercoralis
  • Enterocytozoon spp
  • Hymenolepis spp

Opportunistic Bacteria

  • Bacillus sp.
  • Enterococcus faecalis
  • Enterococcus faecium
  • Morganella sp.
  • Pseudomonas sp.
  • Pseud. aeruginosa
  • Staphylococcus sp.
  • Staph. aureus
  • Streptococcus sp.
  • Methanobacteriaceae
  • Desulfovibrio piger
  • Enterobacter sp.

Pot. AutoImmune Triggers

  • Citrobacter sp.
  • Citrobacter freundii
  • Klebsiella sp.
  • Klebsiella pneumoniae
  • Prevotella copri
  • Proteus sp.
  • Proteus mirabilis
  • Fusobacterium sp.

Fungi & Yeast

  • Candida sp.
  • Candida albicans
  • Geotrichum sp.
  • Saccharomyces cerevisiae.
  • Rhodotorula sp.

Bacterial Pathogens

  • Aeromonas sp.
  • Campylobacter sp.
  • difficile, Toxin A
  • difficile, Toxin B
  • Enterohaemorrhagic E. coli
  • Enteroinvasive E. coli/Shigella
  • Enterotoxigenic E. coli LT/ST
  • Shiga-like toxin E. coli stx 1
  • Shiga-like toxin E. coli stx 2
  • Salmonella sp.
  • Vibrio spp
  • Yersinia enterocolitica
  • Helicobacter pylori PCR & EIA
  • pylori virulence factors (x8)
Viral Pathogens
  • Adenovirus 40/41
  • Norovirus GI/II
  • Rotavirus
  • Sapovirus (I,II,IV,V)
  • Astrovirus (hAstro)

Normal Bacteria/Flora

  • Bacteroides fragilis
  • Bifidobacterium sp.
  • Bifidobacterium longum
  • Enterococcus species
  • Lactobacillus species
  • Lactobacillus rhamnosus
  • Clostridium sp.
  • Enterobacter sp.
  • Akkermansia muciniphila
  • Faecalibacterium prausnitzii
  • Roseburia sp.

Macroscopy

  • Stool Colour
  • Stool Form
  • Mucous
  • Occult Blood

Short Chain Fatty Acids

  • Butyrate
  • Acetate
  • Propionate
  • Valerate

GIT Functional Markers

  • Calprotectin
  • Pancreatic Elastase
  • Secretory IgA
  • Zonulin
  • b-Glucuronidase
  • Steatocrit
  • Transglutaminase IgA

Microbial Culture Along-side PCR (Dual Method)

  • Bacteria
  • Yeasts

Antimicrobial Sensitivities

  • Antibiotics
  • Natural
  • Antifungals
  • Natural Antifungals



PCR

We use real-time polymerase chain reaction (RT-PCR) tests to qualitatively detect nucleic acid from various parasites, bacteria, and viruses. The RT-PCR platform is FDA-authorized and requires specialized equipment and personnel to identify DNA within cells. Polymerase chain reaction (PCR) is a relatively simple and widely used molecular biology technique to amplify and detect DNA and RNA sequences.

The use of PCR testing allows US BioTek to more precisely identify even the smallest amounts of markers within the stool providing accurate, reliable, and reproducible results.

 

ELISA

An established immunological technique, ELISA is widely used to detect the presence of specific antibodies or antigens in a patient’s blood. It is a basic testing platform in a fully-automated protocol utilizing advanced robotics and barcoding to identify each specimen throughout the testing procedure. Antigen-specific antibodies, bound to the antigen, are detected through spectrophotometric analysis.

 

Macroscopy

Stool macroscopy is a method of visually examining stool samples to detect abnormalities such as blood, mucus, parasites, and other foreign materials. The stool is first inspected for color, consistency, and any unusual features.

 

Culture

Stool culture is a laboratory test that identifies bacteria in stool specimens. A small amount of stool is placed on a growth medium and incubated at a warm temperature. If bacteria are present, they will grow and form colonies. The colonies can then be identified by their appearance and by biochemical tests.

Specimen Requirements

Stool Tubes = 2

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