How to collect a dried blood spot sample


Watch the Video: How to use our dried blood spot collection card.

Convenient collection with a simple finger stick.

General Guidelines

  • Each blood collection card and lancet is for one-time use only.
  • Avoid strong repetitive pressure (‘milking’) on the pricked finger. This may cause hemolysis or tissue-fluid contamination of the specimen.
  • Do not rub or smear the blood sample.
  • Air dry only.
  • Missing or incomplete information on the test requisition form will delay the processing time of your specimen.
  • Allow a sufficient quantity of blood to soak through to completely fill in the preprinted circle on the filter paper.

Test kit contents:

  • 2 Alcohol Swabs
  • 2 Lancets
  • Blood Spot Collection Card
  • 2 Bandages
  • 1 Non-Latex Glove
  • 1 Resealable Bag
  • 1 Desiccant
  • 1 Prepaid Priority United States postal Services box ( For USA Shipping)
    1 Prepaid FedEx Billable Stamp (2nd Day Delivery), 1 FedEx Clinical Pak
  • 1 Prepaid Return Envelope. ( For International Shipping)
  • 1 Test Requisition Form

    Out of state specimen kit contains: 

Collection Instructions:

Follow Instructions Carefully. Download instructions PDF here.


  • Complete the patient section on the Requisition Form
  • Print name, date of birth, and the date of collection on the Blood Spot Collection Card. Please ensure this information matches the Requisition Form.
  • Unfold the cover to expose the Blood Collection Pad (hold the card without touching the collection circles on the pad).
  • Continue the fold as you peel away from the back of the Blood Collection pad, exposing the back of the Blood Collection Pad.
  • Make a fold as indicated in the image. Note: It is important that the blood is not in contact with the cover.
    Tip: Taping the outer cover to the edge of a tabletop may help stabilize the Blood Collection Card.
  • The Test Requisition Form must also include:
    • Physician’s Signature
    • Tests Requested
    • Billing Information
    • Specimen Ship Date


  • Wash hands thoroughly with soap and warm water, then dry hands.
  • Use the provided alcohol pad to clean the tip of the finger (ring finger or middle finger).
  • Twist off the blue tab to break the seal on the lancet.
  • Position the lancet against the inside tip of the finger. Press down firmly until an audible click is heard.
  • Gently press the pricked finger from below the puncture site to allow for a large drop of blood to form.
    Important: Wipe away the first drop of blood with provided gauze.
  • Apply a hanging drop of blood. Apply the blood drops within each circle on the Blood Spot Collection Card.


  • Air dry completely. Prop the card up and let air dry for at least 3 hours.
  • Keep specimen away from moisture and sunlight.
  • Once fully dried, tuck the cover into flap as indicated. Insert the Blood Spot Collection Card and desiccant into the resealable bag and seal tightly. Each resealable bag holds a maximum of 5 cards).
    Important: Do not fold the Blood Spot Collection Card.


  • Use the shipping supply provided in the package to return shipment.
  • Specimen may be safely shipped at ambient temperature.
  • US BioTek Laboratories must receive specimen within 55 days at ambient temperature from date of collection.

Diet & Medications

It is suggested that you maintain your usual dietary habits and consume a variety of foods when possible within a couple weeks prior to blood collection, unless advised otherwise by your healthcare practitioner.

Avoid eating foods that may have potentially resulted in a previous adverse reaction and/or anaphylaxis.

Immunosuppressive drugs such as oral or intranasal corticosteroids (e.g. prednisone, beclomethasone, fluticasone, triamcinolone) and topical cortisone suspensions and creams, in addition to immune-modulating drugs, may affect test results for antibody assessment.

Medications: We strongly recommend consulting your healthcare practitioner prior to making any changes to your current medication regimen.